AFP ELISA Rapid Test Kit

AFP ELISA Rapid Test Kit

Singclean AFP ELISA Rapid Test Kit yog rau kev ntsuas ntau ntawm Alpha-fetoprotein (AFP) cov ntsiab lus hauv tib neeg cov ntshav.
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SingcleanAFP ELISA Rapid Test Kityog npaj rau kev txiav txim siab ntau ntawm Alpha-fetoprotein (AFP) cov ntsiab lus hauv tib neeg cov ntshav.

AFP ELISA Rapid Test Kit

Kev qhia tshwj xeeb

48 xeem / khoom siv, 96 xeem / khoom siv

Cov txheej txheem sim

Tso cai rau tag nrho cov tshuaj reagents kom sov hauv chav ua ntej siv:

1. Dilute wash buffer (20 ×) (1:19) nrog dej distilled.

2. Sau daim ntawv lo lossis cim cov kab me me kom siv tau ntawm lub phaj.

3. Pipet 50μL ntawm AFP tus qauv (0,5,20,50,200,500 ng/mL), AFP diluent (cov neeg siv raws li muab), thiab kuaj cov ntshav mus rau hauv qhov dej tsim nyog, sib xyaw kom zoo, npog lub phaj thiab ua kom sov ntawm 37 ° C rau 30 feeb.

4. Aspirate thiab ntxuav lub qhov dej 3 zaug nrog ntxuav tsis huv. Peb pom zoo siv lub tshuab ntxhua khaub ncaws ELISA tsis siv neeg kom zoo dua qub.

5. Ntxiv 100uL ntawm anti-AFP-HRP conjugate reagent rau hauv txhua lub qhov dej. Npog lub phaj thiab muab tso rau ntawm 37 ° C rau 30 feeb.

6. Aspirate thiab ntxuav lub qhov dej 3 zaug nrog ntxuav tsis zoo li saum toj no.

7. Muab 100μL HRP substrate tov rau hauv txhua lub qhov dej. Sib tov maj mam - npog lub phaj thiab muab tso rau 10 min ntawm 37 ℃.

8. Ntxiv 50μL nres kev daws rau txhua qhov dej thiab sib tov maj mam.

9. Nyeem qhov ntom ntom ntom ntom ntom ntom (OD) tus nqi ntawm 450 nm hauv Elisa Analyzer tsis pub dhau 30 feeb tom qab ntxiv qhov kev daws teeb meem nres.

10. Tus qauv nkhaus thiab kab zauv rov qab tau tsim los ntawm AFP tus qauv

xim siv tawv , tom qab ntawd suav qhov AFP concentration hauv cov qauv (Correlation coefficient (r) .990.9900).

Ib txwm siv ntau yam

Ib txwm siv ntau yog ≤ 20ng/mL. Pom zoo lub chaw soj nstuam txhawm rau tsim lawv tus kheej ib txwm siv ntau, cov ntaub ntawv saum toj no tsuas yog siv rau.

Kev txhais lus txhais

Yog tias qhov piv txwv siab dua qhov nrhiav pom txwv, nws muaj peev xwm ua kom dilute nrog AFP diluent. Kev Tshawb Fawb Kev Ntsuas x Dilute Ntau=Thawj Qhov Tseeb

Hu rau peb tam sim no rau SingcleanAFP ELISA Rapid Test Kitnrog tus luv nqi tshwj xeeb!


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